1Medical Microbiology & Pharmocology Unit, Department of Marine Science, Bharathidasan University, Tiruchirappalli, Tamil Nadu, India
2Department of Packaging, Yonsei University, Gangwondo 220-710, Republic of Korea
3Environmental Microbiology and Toxicology Laboratory, Department of Environment Management, Bharathidasan University, Tiruchirappalli, Tamil Nadu, India
*Corresponding author: Manoharan N, Medical Microbiology & Pharmacology Unit, Department of Marine Science, Bharathidasan University, Trichirappalli 24, India
Submission: March 05, 2018; Published: April 27, 2018
The discovery of new broad spectrum antibiotics is urgent need to combat frequently emerging multi drug resistant pathogens (MDR). Actinomycetes, the most important group of microorganisms isolated from marine sources of the world may be the conclusive solution to this problem. Thus the aim of present study was to isolate and identify many secondary metabolite producing actinomycetes from algae sources. The 20 strains of endophyticactinomycetes were screened from marine green algae Cauler pataxifolia and the surface sterilization was proved, the isolates were internal tissue of the algae. Among the 20, 5 strains have better antibacterial activity against multi drug resistant uropathogens by agar well diffusion method. Interestingly, DMS 3 showed excellent inhibition activity against all uropathogens by various solvent systems. Among the various solvent systems, ethyl acetate was exhibited excellent inhibitory activity and showed 24, 27, 20, 19, 16mm zone against E. coli, P. aeroginosa, K. pneumonia, Enterobacter sp respectively. The MIC and MBC also confirmed that the actinomycete strain DMS 3 as excellent antibiotic producer against MDR. It inhibits MDRs of uropathogens at very lowest concentration (100μg/ml).
Keywords: Urinary tract infections; Gram negative bacteria; Multi drug resistant bacteria; Minimum inhibition concentration; Endophytic actinomycetes