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Environmental Analysis & Ecology Studies

Agricultural Applications of Penicillium Genera

Landa Faz A

Department of Biotechnology and Bioengineering, Mexico

*Corresponding author: Landa Faz A, Department of Biotechnology and Bioengineering, CINVESTAV Zacatenco, Av. Instituto Politécnico Nacional 2508, San Pedro Zacatenco, Del. Gustavo A. Madero, 07360, Mexico City, Mexico

Submission: June 25, 2021; Published: April 08, 2022

DOI: 10.31031/EAES.2022.09.000724

ISSN 2578-0336
Volume9 Issue5

Abstract

Penicillium genera are one of the well-known fungi present in many environments since saline soils to artic environments which makes them a microorganism of great interest for different investigations. These microorganisms have been widely studied for food and pharmaceutical applications; However, in recent years it has been seen that these species could have an importance for agricultural applications. These applications include phosphate solubilizing capacity and endophytic properties, which making crops resistant to diseases and extreme environmental conditions; as well as promoting a better crop development. Also, Penicillium species could degrade different xenobiotic compounds like pesticides, phenol, pyrene, crude oil, between other; but a big amount of these researches were made in liquid medium, for that reason the soil applications are a very broad field of research for future investigations. All these characteristics makes the Penicillium genera an interesting topic for future investigations in agricultural and environmental applications.

Keywords: Penicillium; Agricultural application; Phosphate solubilization; Endophytic; Xenobiotic degradation

Introduction

The genera Penicillium are one of the older, well-known and most common fungi that can develop in a diverse range of habitats like soil, air, indoor environments, and food [1]. These fungi are widely studied for its secondary metabolites production, capable of producing substance with important pharmaceutical applications like antimicrobial agents where its major apport was the production of penicillin [2]; but also, its metabolites could be used as immunosuppressants, cholesterol-lowering agents, anti-HIV and antitumor drugs [3]. Also, on food industry has a big importance to produce cheeses, like Camembert or Roquefort [4].

Penicillium species has been reported in many terrestrial environments; up to 200 species have been described in habited soils [5]; and many others have been found at rhizospheric level [6], so it is assumed that this ascomycete has an important role for agricultural applications. To study Penicillium species is also of great interest, because they can develop under extreme conditions, like 4 °C, pH ranges from 2 to 12 and salinity up to 20% w/w [7]; also, different species have been isolated from unconventional environments such as the Arctic, mines, and ocean sediments [3]. Given its versatile development are microorganism interesting to study, for environmental and agricultural applications.

Discussion

Phosphate solubilization

Probably the phosphates solubilization is the best known and most useful agricultural application of Penicillium species. Phosphorus is an element that promotes plant growth, and less than 10% of the phosphorus in the soil is available for the biogeochemical cycle [7]. This property has been associated with the segregation of organic acid’s such as tricarboxylic, citric and oxalic, among others [8]. The function of these acids is the acidification of soils, allowing the availability of micronutrients, such as Fe, Zn and Mn.

The organic acid production has another importance, associated to microbial chemotaxis and metal detoxification [9], which represent another benefit to use Penicillium species in soil. A big amount of conventional fertilizers has traces of Cadmium [10] which is a heavy metal associated to Anemia, Kidney, Lung and Bone Damage; as well as Nervous Disorders and Cancer [11] so the use of solubilizing microorganisms instead of fertilizers shows an environmental and health impact, in order that is possible to reduce the periodicity of fertilization, as well as the concentration of these compounds. The above implications suggest that the use of Penicillium species instead of chemical fertilizers for its capacity of solubilize phosphorus, could be an interesting research topic for future investigations.

Endophytic fungus

Another important and useful application of the Penicillium genera is associated with its ability to act as an endophytic fungus in different crops, which involves [12]:

A. Greater access to nutrients to the crop.

B. Protection against extreme conditions.

C. Protection against insects, parasites, and other microbes.

Protection against pathogen organism: The resistance to insects and other organism happens when crops are expose to Penicillium species. It generates a type of resistance to pathogens, which is known as a Systematic Acquired Resistance (SAR), when it happens the crop activates different enzymes (Chitinases, Glucanases, Peroxidases, Oxidases and Lyases), metabolites (Salicylic and Jasmonic Acids) and phenolic compounds as a defense mechanism which protect them from attacks of pathogenic organisms [13].

There are some works related to pathogen resistance of crops when were in contact with different species of the genera Penicillium, Table 1 present one of these investigations. Another action mechanism of Penicillium species as protection of pathogen organism, is related to secondary metabolites production like penintrems. An example of this, is the penintrem A production by P. crustosum that showed a 100% reduction in adults of milkweed inche (Oncopeltus fasciatus); as well as penintrem C which showed a reduction of 73.3% for the same organism, and 100% for adult fruit flies (Ceratitis capitata) at a dose of 10μg/fly [18]. All these results shows that the application of Penicillium species, as a biological control instead of chemical, make a healthier and ecofriendly crop production.

Table 1: Protection of different Penicillium species against pathogen organism in different plants.


Crop improvement: As was mentioned in section 4.2, endophytic fungi also providing greater access of soils nutrients and this, together with the secretion of hormones, is reflected in a better crop development; Table 2 shows some examples of this behavior. These results, together with the phosphate solubilizing capacity mentioned before, make the use of Penicillium genera as a biofertilizer of great interest for the development of organic crops [19-22].

Table 2: Crop improvement by different Penicillium species.


Degradation of xenobiotic compounds

Another application of great interest is related to the capacity of Penicillium species to degrade different xenobiotic compounds; this behavior is associated to the productions of reactive oxygen species which oxidate the compound [22] in one less toxic, and in some cases the complete mineralization (CO2 and water). A big amount of these researches were made in liquid medium, but there are some works in soil systems that are present in Table 3. The fact that Penicillium species can be used as biofertilizers, but also have the ability to biodegrade xenobiotic compounds, makes them a microorganisms of highly important for agricultural applications, which have not been fully study [23-28].

Table 3: Penicillium species for degradation of different xenobiotics in soil.


Conclusion

Given the excessive use of agrochemicals, looking to stop pests, and increasing soil fertility, in recent years, we are consumed vegetables of low nutritional and health qualities; as was previously discuss an alternative to improve crop development and make them resistant to diseases and extreme environmental conditions could be the use of Penicillium species. These genera can also, biodegraded different xenobiotics compounds like pesticides, phenol, pyrene, crude oil, between other, which makes these microorganisms interesting species to study for future environmental and agricultural investigations.

Acknowledgement

CONACYT PhD. scholarship 306251 for Anbu Landa-Faz.

References

  1. Visagie CM, Houbraken J, Frisvad JC, Hong SB, Klaassen CHW, et al. (2014) Identification and nomenclature of the genus Penicillium. Stud Mycol 78: 343-371.
  2. Fleming A (1929) On the Antibacterial Action of Cultures of a Penicillium, with Special Reference to their Use in the Isolation of influenzæ. Br J Exp Pathol 10(3): 226-236.
  3. Koolen HHF, Soares ER, Silva FMA, Almeida RA, Souza ADL, et al. (2012) An antimicrobial alkaloid and other metabolites produced by Penicillium sp. An endophytic fungus isolated from Mauritia flexuosa L. F. Química Nova 35(4): 771-774.
  4. Giraud F, Giraud T, Aguileta G, Fournier E, Samson R, et al. (2010) Microsatellite loci to recognize species for the cheese starter and contaminating strains associated with cheese manufacturing. Int J Food Microbiol 137(2-3): 204-213.
  5. Petit P, Lucas EM, Abreu LM, Pfenning LH, Takahashi JA (2009) Novel antimicrobial secondary metabolites from a Penicillium sp. isolated from Brazilian Cerrado Soil. Electron J Biotechnol 12(4): 8-9.
  6. Phuwiwat W, Soytong K (2001) The effect of Penicillium notatum on plant growth. Fungal Divers 8(1): 143-148.
  7. Pandey A, Das N, Kumar B, Rinu K, Trivedi P (2008) Phosphate solubilization by Penicillium spp. isolated from soil samples of Indian Himalayan region. World J Microbiol Biotechnol 24(1): 97-102.
  8. Fenice M, Selbman L, Federici F, Vassilev N (2000) Application of encapsulated Penicillium variabile P16 in solubilization of rock phosphate. Bioresour Technol 73(2): 157-162.
  9. Paredes MM, Espinosa VD (2010) Organic acids produced by phosphate solubilizing rhizobacteria: A Critical Review. Terra Latinoamericana 28(1): 61-70.
  10. Grant CA, Bailey LD, Harapiak JT, Flore NA (2002) Effect of phosphate source, rate and cadmium content and use of Penicillium bilaii on phosphorus, zinc and cadmium concentration in durum wheat grain. J Sci Food Agric 82(3): 301-308.
  11. Londoño FLF, Londoño MPT, Muñoz GFG (2016) The risks of heavy metals in human and animal health. Biotechnology in the Agricultural and Agroindustrial sector 14(2): 145-153.
  12. Vega FE, Posada F, Peterson SW, Gianfagna TJ, Chaves F (2006) Penicillium species endophytic in coffee plants and ochratoxin A production. Mycologia 98(1): 31-42.
  13. Koike N, Hyakumachi M, Kageyama K, Tsuyumu S, Doke N (2001) Induction of systemic resistance in cucumber against several diseases by plant growth-promoting fungi: Lignification and superoxide generation. Eur J Plant Pathol 107(5): 523-533.
  14. Elsharkawy MM, Shimizu M, Takahashi H, Hyakumachi M (2012) Induction of systemic resistance against Cucumber mosaic virus by Penicillium simplicissimum GP17‐2 in Arabidopsis and tobacco. Plant Pathol 61(5): 964-976.
  15. Hossain MM, Sultana F, Kubota M, Koyama H, Hyakumachi M (2007) The plant growth-promoting fungus Penicillium simplicissimum GP17-2 induces resistance in Arabidopsis thaliana by activation of multiple defense signals. Plant Cell Physiol, 48(12): 1724-1736.
  16. Thuerig B, Felix G, Binder A, Boller T, Tamm L (2005) An extract of Penicillium chrysogenum elicits early defense-related responses and induces resistance in Arabidopsis thaliana independently of known signalling pathways. Physiol Mol Plant Path 67(3-5): 180-193.
  17. Thuerig B, Binder A, Boller T, Guyer U, Jiménez S, et al. (2006) An aqueous extract of the dry mycelium of Penicillium chrysogenum induces resistance in several crops under controlled and field conditions. Eur J Plant Pathol 114(2): 185-197.
  18. González MC, Lull C, Moya P, Ayala I, Primo J, et al. (2003) Insecticidal activity of penitrems, including penitrem G, a new member of the family isolated from Penicillium crustosum. J Agric Food Chem 51(8): 2156-2160.
  19. Phuwiwat W, Soytong K (2001) The effect of Penicillium notatum on plant growth. Fungal Divers 8(1): 143-148.
  20. Bhagobaty RK, Joshi SR (2009) Promotion of seed germination of Green gram and Chick pea by Penicillium verruculosum RS7PF, a root endophytic fungus of Potentilla fulgens L. Advanced Biotech 8(7): 16-18.
  21. Landa Faz A, González Orenga S, Boscaiu M, Rodríguez VR, Vicente O (2021) Effect of the Pesticide Endosulfan and Two Different Biostimulants on the Stress Responses of Phaseolus leptostachyus Plants Grown in a Saline Soil. Agronomy 11(6): 1208.
  22. Izcapa TC, Loera O, Tomasini CA, Esparza GF, Salazar MJA, et al. (2009) Fenton (H2O2/Fe) reaction involved in Penicillium sp. culture for DDT [1, 1, 1-trichloro-2, 2-bis (p-chlorophenyl) ethane)] degradation. J Environ Sci Health Part B 44(8): 798-804.
  23. Mohanasrinivasan V, Suganthi V, Selvarajan E, Subathra Devi C, Ajith E, et al. (2013) Bioremediation of endosulfan contaminated soil. Res J Chem Environ 17: 93-101.
  24. Bisht J, Harsh NSK, Palni LMS, Agnihotri V, Kumar A (2019) Biodegradation of chlorinated organic pesticides endosulfan and chlorpyrifos in soil extract broth using fungi. Remediation J 29(3): 63-77.
  25. Landa Faz A, Rodríguez VR, Roldán Carrillo TG, Hidalgo Lara ME, Aguilar López R, et al. (2021) Bioremediation of an agricultural saline soil contaminated with endosulfan and Escherichia coli by an active surface agent induced in a Penicillium crustosum culture. Prep Biochem Biotechnol 52(3): 292-301.
  26. Scow KM, Li D, Manilal VB, Alexander M (1990) Mineralization of organic compounds at low concentrations by filamentous fungi. Mycological Research 94(6): 793-798.
  27. Pinto LJ, Moore MM (2000) Release of polycyclic aromatic hydrocarbons from contaminated soils by surfactant and remediation of this effluent by Penicillium spp. Environ Toxicol Chem 19(7): 1741-1748.
  28. Nrior RR, Onwuka NF (2017) Bioremediation of crude oil contaminated marshland muddy soil by bioaugmentation approach using Candida tropicalis and Penicillium chrysogenum. Environ Sci Toxicol Food Technol 11(10): 57-64.

© 2022 © Landa Faz A. This is an open access article distributed under the terms of the Creative Commons Attribution License , which permits unrestricted use, distribution, and build upon your work non-commercially.

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