Crimson Publishers Publish With Us Reprints e-Books Video articles

Full Text

Environmental Analysis & Ecology Studies

Phytochemical Characterization of the Species Thuja columnaris Short Communications

Hiticaş VV, Mîndruleanu A, Ioan Sarac and Monica Butnariu*

King Michael I of Romania from Timisoara, Romania

*Corresponding author: Monica Butnariu, King Michael I of Romania from Timisoara, Romania

Submission: January 19, 2019;Published: October 18, 2019

DOI: 10.31031/EAES.2019.06.000637

ISSN 2578-0336
Volume6 Issue3

Abstract

Thuja columnaris has been used in traditionality medicine for the treatment of rheumatism, amenorrhea, cystitis, and uterine carcinomas, and as an abortifacient and contraceptive. Several plant-derived essential oils have been known for over a century to have epileptogenic properties. A survey of the literature shows essential oils of 11 plants to be powerful convulsants (fennel, eucalyptus, pennyroyal, hyssop, sage, rosemary, tansy, savin, turpentine, thuja, and wormwood) due to their content of highly reactive monoterpene ketones, such as camphor (C10H16O), pinocamphone (C10H16O), thujone (C10H16O), cineole (C10H18O), pulegone (C10H16O), sabinyl acetate (C12H18O2), and fenchone (C10H16O).

Keywords: Thuja columnaris; Essential oils; Antimicrobial activity

Background

The essential oils from leaves, twigs and stems of large trees and shrub-like trees of Thuja columnaris were extracted by hydrodistillation and supercritical fluid extraction, analyzed by chromatographic methods [1].

The essential oil composition differed significantly among the three organs, as well as between large trees and shrub-like trees [2]. Furthermore, many differences in the essential oil composition between Thuja columnaris and other Thuja species were apparent [3]. The essential oils exhibited a certain degree of antifungal activity against strains of human pathogenic fungi [4]. Chemical composition and pharmacological activity: The main compounds of the oil were the monoterpene ketones α- and β-thujone, fenchone, and sabinene, as well as the diterpenes beyerene and rimuene [5]. The neurotoxic thujones (α- and β-diastereoisomers) are common compounds of Thuja columnaris plant essential oils. Thuja columnaris has many pharmacological properties but has no anti-inflammatory activity, and contents 54.78-11.28% alpha-pinene (C10H16) and (+)-4-carene (C10H16), 6.87% alphacedrol (C15H2O), 5.88% terpinolene (C10H16), 5.21% p-menth-1-en-8-ol acetate (C12H20O2), 4.04% beta-myrcene (C10H16), 2.26% beta-pinene (C10H16), 1.72% germacrene D (C15H24), 1.65% sabinene (C10H16) and 1.62% D-Limonene (C10H16) [6].

The fruit oil contained 52.4% alpha-pinene (C10H16), 14.2% delta-3-carene (C10H16), 6.5% alpha-cedrol (C15H2O) and 5.1% beta-phellandrene (C10H16), the leaf oil contained 21.9% alpha-pinene (C10H16), 20.3% alpha-cedrol (C10H16), 10.5% delta-3-carene (C10H16) and 7.2 % limonen (C10H16) as the main components [7]. The leaf oil contained 29.2% alpha-pinene (C10H16), 20.1% Delta-3-carene (C10H16), 9.8 % alpha-cedrol (C15H2O), 7.5% caryophyllene (C15H24), 5.6% alpha-humulene (C15H24), 5.4% limonene (C10H16), 3.8% alpha-terpinolene (C10H16) and 3.5% alpha-terpinyl acetate (C12H20O2) as major compounds [8]. A phytochemical investigation on the essential oil of Thuja columnaris resulted in the isolation and identification of three sesquiterpenes, 3α-methoxy-4α-epoxythujopsane, Δ³⁵-4β-epoxythujopsene, and Δ³⁴- thujopsen-2,15-diol, together with eight known sesquiterpenoids. The compounds, beyerene and the mixture of alpha-thujone and beta-thujone, were isolated from the oils and tested against Gram-positive and negative bacteria and pathogenic fungi. The oils of the Thuja columnaris species exhibited significant antimicrobial activity, while the mixture of alpha- and beta-thujone showed very strong activity as well [9].

Conclusion

T. columnaris essential oil and its active component, α-thujone, can be used for the treatment of polycystic ovary syndrome without inducing osteoporosis. Nowadays the wide use of these compounds in certain unconventional medicines make this severe complication again possible.

References

  1. Ojogu NA, Annune PA, Okayi GR (2017) Toxicological effects of aqueous extract of piptadeniastrium africanum bark on Clarias gariepinus juveniles. Banat's Journal of Biotechnology 8(15): 123-135.
  2. Ghasemi E, Kohnehrouz BB (2016) Cloning the cotton rrn23-rrn5 region for developing a universal interfamily plastidial vector. Banat's Journal of Biotechnology 7(14): 81-88.
  3. Ould Yerou K, Meddah B, Touil AT, Sarsar F (2017) Laurus nobilis from algeria and immune response. Banat's Journal of Biotechnology 8(15): 119-122.
  4. Ruthin AB (2017) The effects of illumination on the early development of tailed and tailless amphibians. Banat's Journal of Biotechnology 8 (15): 113-118.
  5. Rezaei A, Akhshabi S, Sadeghi F (2016) Evaluation of exon 17 of insulin receptor (INSR) gene and its relationship with diabetes type 2 in an Iranian population. Banat's Journal of Biotechnology 7(13): 61-69.
  6. Salajegheh AMM, Ahmadimoghadam A, Mirtadzadini SM (2017) Distribution of cyanobacteria in two sirch hot springs with regards to the physicochemical traits of water. Banat's Journal of Biotechnology 8(15): 83-89.
  7. Basuny AMM, Al Oatibi HH (2016) Effect of a novel technology (air and vacuum frying) on sensory evaluation and acrylamide generation in fried potato chips. Banat's Journal of Biotechnology 7(14): 101-112.
  8. Bhattacharya A, Sadhukhan AK, Ganguly A, Chatterjee PK (2016) Investigations on microbial fermentation of hemicellulose hydrolysate for xylitol production. Banat's Journal of Biotechnology 7(14): 13-23.
  9. IDRIS A (2016) Comparative analysis of 16SrRNA genes of Klebsiella isolated from groundnut and some American type culture collections, Banat's Journal of Biotechnology 7(13): 34-40.

© 2019 Monica Butnariu. This is an open access article distributed under the terms of the Creative Commons Attribution License , which permits unrestricted use, distribution, and build upon your work non-commercially.

About Crimson

We at Crimson Publishing are a group of people with a combined passion for science and research, who wants to bring to the world a unified platform where all scientific know-how is available read more...

Leave a comment

Contact Info

  • Crimson Publishers, LLC
  • 555 Madison Avenue, 5th floor
  •     New York, NY 10022, USA
  • +1 (929) 600-8049
  • +1 (929) 447-1137
  • info@crimsonpublishers.com
  • www.crimsonpublishers.com