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Environmental Analysis & Ecology Studies

Occurrence of Fungicide Oxadixyl In the Surface Water of an Urban Lake

Villanueva JD1,2*, Le Coustumer P2, Peyraube N3, Granger D4, Thiennot R4 and Ley L5

1University of the Philippines, SESAM-UPLB College, Philippines

2Université de Bordeaux, EA 4592 Géoressources & Environnement, France

3Université de Bordeaux, UMR 5295 I2M-GCE, France

4LyRE, Lyonnaise des Eux, France

5Laboratoire Départemental D’Analyse et de Recherche, Dordogne (LDAR 24), France

*Corresponding author: Villanueva JD, University of the Philippines, SESAM- UPLB, College, Philippines

Submission: September 25, 2018;Published: October 18, 2019

DOI: 10.31031/EAES.2019.06.000636

ISSN 2578-0336
Volume6 Issue3


The ongoing re-emerging Nipah Virus (NiV) outbreak represents serious public and global health concern with 12 deaths including 3 laboratories confirmed, and over 25 suspected cases in Kozhikode district, Southwest coast of India. Overall, more than 100 deaths out of over 600 reported human cases have been reported since 1998 mainly in Bangladesh, Malaysia, Singapore and elsewhere. Fostering R&D and operational research priority on NiV and risk factors mapping in forecasting and modelling in improving further R&D investment for better communities’ preparedness and similar to Ebola and SARS viruses’ outbreaks threats and consequences. NiV outbreak R&D roadmap leadership and investment is crucial to ensure availability of diagnostic tools, accurate and timely safe drugs NiV/HeV infection or vaccine for scale immunization in endemic areas in addition to community awareness and training, health education and resilience programs is vital to increase the likelihood and sustainable development.

Keywords:Nipah virus; Outbreak; Roadmap; R&D; Vaccine, Alertness; Capacity building India; Bangladesh; Malaysia; Singapore


Oxadixyl (C14H18N2O4) is a phenylamide fungicide [1]. This fungicide is used to treat diseases caused by Oomycetes family [2]. It was employed since 1986 for various purposes such as protection of barley, potatoes and vegetables (Lettuce, Carrot, Cabbage, Spinach, etc.), treatment of ornamental tree seeds and treatment of the soils. In Southwest of France, it was specifically used against mildew, a common disease seen in the vineyards. The use of Oxadixyl in France was prohibited since year 2003 as it was found toxic for the environment and for humans. According to the European Regulation of 1907/2006/EC, Article 31, Oxadixyl is toxic to aquatic life with long lasting effect. For humans, it is harmful if swallowed and inhaled, and in contact with skin and eyes.

As a systemic pesticide, it implies environmental distresses. Systemic pesticide persists in soil for long duration that reaches groundwater, resulting to contamination [3]. Another type of risk in water is that Oxadixyl slowly hydrolyzes that can release ammonia and form acetate salts [4]. In the United States, product cancellation was earlier as it started year 2002. The aim of this study is to find out if Oxadixyl is present in the surface water of Bordeaux Lac in France. This lake is important for its recreational use (swimming area and sailing school) and habitat for aquatic resources. Lakes are prone to organic contamination [5] due mostly to different agricultural [6] and industrial activities [7]. The occurrence of organic molecule in aquatic systems should be assessed to determine the state of the water quality [8]. Sampling campaigns were done in 2011, 8 years after the banning was implemented.

Site background

Bordeaux Lac is situated in Southwest of France, Northern part of Bordeaux City. It is manmade, used to be a swamp area, dug during 1960 with a surface area of 150ha. Its depth can extend to around 15m deep. Water comes from the local water table. This lake is a home to aquatic species such as lacustrine and cyprinids. It caters recreational activities such as fishing, swimming every summer, and nautical service such as sailing school. This lake accommodates surface water runoff channeled by a collector. Climate of Bordeaux area is temperate, with oceanic influence. Monthly average temperature ranges from 6.6°C in January to 21.4 °C in July (measures from 1981 to 2018). Annual accumulated rainfall is approximately 950mm.


Sampling sites

Four (4) sampling sites were chosen (Erreur! Source du renvoi introuvable.gure 1). Sampling site 1 is at the collector, discharging surface runoff on urban area (mainly the nearby highway) directly to the lake. Sampling sites 2 and 3 were collected approximately at the center of the lake with 1m and 6m depths, respectively. Sampling Site 4 is at the sailing school.

Two sampling campaigns were done in summer 2011: the first is held from 20 June to 4 July, the second is held from 12 August to 26 August. In both cases, samplings are done three times with oneweek interval. Two months before the first sampling campaign was done, the months of March and April did not receive the expected rainfall (average rainfall from 1981 to 2018 are 65mm and 78mm respectively). March had 32.4mm while April had 15.7mm. The scarcity of the rainfall continued while the 1st sampling period was conducted. Accumulated rainfall before the sampling dates is presented in Table 1.

Table 1:Frequency and percentage of rodent hunting in the villages studied in Bastak County.

This season is considered as a dry period. Before the second sampling campaign, a long rainfall period started on the 7th of July with a peak on mid-July (Figure 1). The months of July and August gave rainfall records that are higher than the monthly normal average. Although, the year 2011, was considered a dry year, these months succeeded in having rainfall above the normal monthly average. Thus, this season can be considered as a wet period.

Figure 1:Amastigote of Leishmaniasis major in prepared sample from Meriones hurricane species, Bastak County.

Using the conventional method, collecting water samples was done manually. Each amber bottle was immersed to the water and then immediately covered with a cap (to avoid airborne contaminants). There should be no air bubbles, so it has to be checked. If there is, the procedure should be repeated. Van Dorn water sampler is used for collecting waters with specific depth at the Bordeaux Lac (1 and 6 meters). Water samples were sent to the Laboratoire Départemental d’Analyse et de Recherché de Dordogne (LDAR 24). These were analyzed using the protocol PS 13/14/11 of the Liquid Chromatography Mass Spectrometry (LC-MS/MS).

Result and Discussion

The measured concentration of Oxadixyl in the four sites during the two campaigns is presented in Figure 2. Accumulated rainfall (over 7 days) and daily rainfall are also showed. Eight years after its interdiction of use, Oxadixyl is still present in the aquatic system of Bordeaux Lac. For the first campaign, the concentrations in the four sites were rather low, 43ng/L on the average. Also, the occurrence of Oxadixyl is variable. For example, concentrations were less than 2ng/L for the sampling of 20th of June 2011 for the four sites, but it reached 160ng/L at the collector a week later (27th of June 2011). During the second campaign, the average concentration was 72ng/L with a maximum of 110ng/L at 6m depth on the 26th of August 2011.

Figure 2:Amastigote of Leishmaniasis major in prepared sample from Meriones hurricane species, Bastak County.

The first campaign is following a dry period. Even if the amount of rainfall was lesser, the presence of Oxadixyl was still detected in Bordeaux Lac. This detection indicates that this fungicide reached the surface water. The collector presents strong variations of concentration within a week (first sampling campaign). Yet, it does not seem to be a major contributor of this molecule. The second sampling campaign took place after a rainy period. During this period, the highest concentration was detected at the area of 6 meter deep. Intensity of rainfall and its impact on surface runoff and soil leaching should be investigated. As a phenylamide, its persistence could be expected to maintain stability in the aquatic environment [9]. The study of Aldana et al. [10] showed that Oxadixyl is mobile in soil columns and leaching can be highly expected. If this is the case, the stock of Oxadixyl in the soil and its surrounding could be mobilized.

Occurrence of Oxadixyl in Bordeaux Lac water is a concern for the health of the people and its aquatic system’s biota. Although the concentrations are low, monitoring of this molecule is needed. The area is used as for a recreational activity (e.g. swimming, fishing). People who use this area have the risk of being exposed to this molecule. Water swallowing might be experienced. The origin and transportation processes of Oxadixyl are still not known.

Soil samplings are needed to better estimate the remaining stocks of Oxadixyl. Also, the role of the collector should be assessed, especially the occurrence of high concentrations. The two campaigns showed a general behavior influenced by the rainfall. However, 20 June 2011 and 26 August measurements have different variations despite of the fact that they are following rain event (6mm on 18 June 2011, 16mm on 24 August 2011). Then, the effects of rain event intensity as well as the humidification state of the soil should be addressed. The first sampling campaign showed that during the dry period (when the precipitation is low), the concentration is low, but a peak or highest concentration was detected at the collector.

While during the wet period, in August the pronounced rainfall was experienced. The concentration of the fungicide can be washed out or diluted. Hence, in general concentration can be expected low. Intensity and frequency of the occurrence of rainfall have impact on the concentration of an element or molecule [11]. The highest concentration in the collector during the 1st sampling campaign and at the center of 6-meter-deep during the 2nd sampling should be further investigated.


The two sampling campaigns conducted in Bordeaux Lac in June and August 2011 showed that Oxadixylis still present in the aquatic system even 8 years after its interdiction. The occurrence of Oxadixyl could pose risks for the aquatic system and for the people using the area. Especially as Bordeaux Lac is used for fishing, sailing and swimming by adults and infants. The measured concentrations in water were variable, ranging from less than 2ng/L to 160ng/L. Even if this concentration is considered low, their detection proves its occurrence in this surface water. Its persistence is observed, albeit, the differing rainfall conditions. Also, its concentration variation founds appealing to measure and assess the mass fluxes. Rainfall seems to be a possible driver of Oxadixyl in terms of concentration variations. Leaching of stocks in the soils could transfer the pollutant to groundwater and later to Bordeaux Lac. However, the transfer processes are not yet clearly assessed. Therefore, monitoring of Oxadixyl in Bordeaux Lac water should be done to better understand the role of rainfall, groundwater and runoff water.


The authors would like to thank the Lyonnaise des Eaux of Bordeaux, France and the European Union ERASMUS MUNDUS External Cooperation Window (ECW) Lot 12/13 for funding this research; and the Bourse Eiffel Excellence (Programme2012-2013) from the French Ministry of Foreign Affairs for providing the academic grant


  1. Mehrotra RS (2013) Fundamentals of Plant Pathology. Tata McGraw6Hill Education. pp. 276.
  2. Roberts TR, Roberts TR, Hutson DH, Jewess PJ (1998) Metabolic Pathways of Agrochemicals: Insecticides and Fungicides. Royal Society of Chemistry. pp. 1269.
  3. Gross M (2014) Systemic pesticide concerns extend beyond the bees. Current Biology 24(16): R717-R720.
  4. Greene SA (2013) Sittig’s Handbook of Pesticides and Agricultural Chemicals. Personal care and cosmetic technology. Edited by William Andrew. pp. 656.
  5. McGoldrick DJ, Murphy EW (2016) Concentration and distribution of contaminant in lake trout and walleye from the Laurentian Great Lakes (2008-2012). Environmental Pollution 217: 85-96.
  6. Challis JK, Cuscito LD, Joudan S, Luong KH, Knapp CW, et al. (2018) Inputs, source apportionment, and transboundary transport of pesticides and other polar organic contaminants along the lower Red River, Manitoba, Canada. Science of the Total Environment 635: 802-816.
  7. Wattigney WA, Irvin-Barnwell E, Li Z, Davis SI, Manente S, et al. (2019) Biomonitoring programs in Michigan, Minnesota and New York to assess human exposure to Great Lakes contaminants. International Journal of Hygiene and Environmental Health. In Press 222(1): 125-135.
  8. Villanueva JD, Allan I, Reid M, Peyraube N, Le Coustumer P (2017) Fate and Transport of Ibuprofen in the Natural Surface Water of the Pasig River, Philippines. International Journal of Environmental Science 2: 248-257.
  9. El Dib MA, OA Aly (1976) Persistenc of some Phenylamide Pesticides in the Aquatic Environment-I. Hydrolysis. Water Research 10: pp. 1050.
  10. Aldana M, De Prado R, Martínez MJ (2011) Leaching of Oxadixyl and Tebuconazole in Colombian Soil. Communications in Agricultural and Applied Biological Sciences, Ghent University 76(4): 909-914.
  11. Villanueva JD, Granger D, Binet G, Litrico X, Huneau F, et al. (2016) Labile trace metal contribution of the runoff collector to a semi-urban river. Environmental Science and Pollution Research 23(11): 11298-11311.

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