1 School of Chinese Materia Medical, China
2 State Key Laboratory of Quality Research in Chinese Medicine, China
3 School of Fundamental Medical Science, China
*Corresponding author: Hongying Cao, School of Chinese Materia Medica, China Ping Huang, School of Chinese Materia Medica, China
Submission: January 09, 2019;Published: January 22, 2019
ISSN: 2637-7802Volume3 Issue4
Context: Suo Quan Wan (SQW) is an effective traditional Chinese prescription for treating lower urinary tract symptoms and exhibits modulatory effects on TRPV1 and P2X3 expression and adenosine triphosphate (ATP) release in accordance with the recovery of bladder function in overactive bladder (OAB) rat. This interaction between cells of the bladder is relayed to gap junction channels.
Objective: This experiment further investigates the mechanism of SQW in modulating gap junction channels by Cx43. Materials and Methods: This study was carried out using a guinea pig OAB model created by bladder outlet obstruction (BOO) surgery. The model was divided into the model group and SQW-treated group with the sham operation group as control. In vivo study with voided stain on paper test was performed to evaluate the micturition behavior of guinea pigs, and urodynamic assessment was conducted to measure the differences in bladder functions of each group. The bladder weight to body weight ratio was also analyzed. Then, real-time polymerase chain reaction and Western blot were performed to quantify TRPV1 expression in the bladder. An In vitro organ bath was used to study bladder distension response to various compounds.
Results: The guinea pigs received BOO surgery after 3 weeks and exhibited significantly increased micturition times, bladder pressure (BP) and capacity, and postvoiding residual. Meanwhile, voiding efficiency (VE) was decreased. In vitro study showed increased force response to α, β-me-ATP, KCl, and carbachol and high expression level of Cx43 on the bladder. The bladder weight to body weight ratio was also increased. SQW treatment can improve bladder function by modulating BP and increasing VE to reduce postvoiding residual, which decreases micturition times. Cx43 expression was decreased. Force response to α, β-me ATP, KCl, and carbachol was decreased.
Conclusion: SQW can modulate Cx43 expression in accordance with the recovery of bladder function.
Keywords: Suo Quan Wan (SQW); Intracellular communication; Connexin 43; Overactive bladder; Outlet obstruction