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Advancements in Bioequivalence & Bioavailability

Classical Drug Serratia Marcescens L-Asparaginase and Anticancer Activity In Vitro

  • Open or Close Rahul Hajare*

    Post Doc Scholar, Indian Council of Medical Research New Delhi, India

    *Corresponding author: Rahul Hajare, Post Doc Scholar, Indian Council of Medical Research, New Delhi, India

Submission: September 23, 2018; Published: September 26, 2018;

DOI: 10.31031/ABB.2018.02.000524

ISSN 2640-9275
Volume1 Issue5


The present study aimed to production of L-asparaginase from spiny cactus peel waste extract (SCPWE) by submerged cultures of Serratia marcescens. S. marcescens grew well for 180h in SCPWE containing medium, revealed the presence of enough carbohydrate source for growing S. marcescens and production of Asparaginase. Different parameters, such as pH [1,2], temperature and incubation period, were optimized for growth and maximum L-asparaginase production. The optimum culture parameters were 30ì 60h and pH 8.6. Maximum L-asparaginase and biomass were observed at the end of the logarithmic phase (6.4 IU/ml and 2.96g%, respectively). L-asparaginase activity and biomass were increased from 1.4IU/ml and 0.56g% to 6.4 IU/ml and 2.96 g% respectively at the end of logarithmic phase (60h). Higher yields of L-asparaginase (6.4IU/ml) with a specific activity of 1984IU/ml (89-fold purification with 39 % recovery) were obtained from S. marcescens cultures. The purified L-asparaginase was used for the characterization and general properties were used such as effect of pH and temperature as well as stability at pH and temperature on L-asparaginase activity.

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