Acute Gastroenterıtıs Agents Under 5 Years Old Age Chıldren

Acute gastroenteritis is one of the most common health problems worldwide [1]. Acute infectious diarrhea is a common disease in young children throughout the world too. Estimated incidence rates in developing countries range from 3.5 to 7.0 episodes per child per year during the first 2 years of life and from 2 to 5 episodes per child per year for the first 5 years [2]. Pediatric diarrhea is a costly disease in terms of direct (and indirect) monetary costs to each community, and it is a cause of emotional trauma for the child and the parents [3].


Materials and Methods
The study group comprised 216 children admitted consecutively for diarrhea to this hospital during a period of one year. The patients were chosen if they had diarrhoea on admission, irrespective of other concomitant diseases. Children who had been treated with antibiotics before the onset of diarrhoea were included too. Samples were obtained by direct deposition in a sterile container and were transported the same day to hospital laboratories, where they were stored at 4-8 ˚C until they were processed. Specimens for bacteriological culture were inoculated into appropriate media on the day of collection.
Stool specimens from each infant with severe gastroenteritis were tested for Clostridium difficile by means of enzyme-linked immunoassay. CerTest Rotavirus and Adenovirus Card Test (CerTest, Biotec, Spain), a qualitative immunochromatographic assay was used to detect Rotavirus and Adenovirus antigens.This immunochromatic tests used for detecting Giardia and Entemoeba antigens too and most of the cases we support our finding with the microscopy. They were also examined by ELISA for Clostridium difficile cytotoxins A and B. We used Macconcey medium for detecting the lactose negative colonies after that Selenit-F media used for detecting only Salmonella and Shigella in the stool samples. We don't find the serotypes of this bacterias. In this study we use IMVIC tests define as symptoms of diarrhoea not attributable to another cause.

Result
The total number of children admitted during the 1 year of survey was 216 children. Rotaviruses were the single most common pathogen. For all agents peak incidence occured in February (n=27) and August (n=28) ( Table 1). Adenoviruses present in 29 of the total number of 216(13.42%) patients. Clostridium difficile strains isolated from 30 of 216(13.88%) ,parasites including ; Giardia and Entemoeba were identified in 19(8.79%) and 26(12.03%)( Table 1).
Some agents are peak in the cold seasons for example most Rotavirus outbreaks increased in winter .(December 14.77%, January 13.76%, February 25%) Looking at all the months, concluded that there isn't significant difference about Adenovirus infections.We found 5 Adenovirus-Rotavirus mix infections 2 case in February , 1 case in March and 3 case in April (Table 1). In contrast some outbreaks occured in hot seasons especially Salmonella (June 21.42%, July 28.57%, August 35.71%) and Shigella (June 20%, July 20%, August 30%) ( Table 1).

Discussion
Acute gastroenteritis in children continues to be a significant problem throughout the world.Worldwide more than 744-1000 million cases of acute diarrhoeal disease are estimated to ocur annually just in children under 5 years old [16]. Of the estimated total 10.6 million deaths among children under five years of age worldwide, 42 percent occur in the World Health Organization (WHO African region) [17]. Throughout the world global estimates of the number of deaths due to diarrhea have shown like [18], from 4.6 million in the 1980s [16] [20].
Some studies refers that enterotoxigenic Rotaviruses predominate in developing areas, cytotoxigenic Clostridium difficile are seen with increasing frequency in developed areas; and Shigella, Salmonella, and Giardia lamblia are found throughout the world [21]. In Netherland viruses were detected in 82% of the Res Med Eng Sci samples, with Rotavirus being most common (56%), bacteria in 32% and parasites in 10% [22]. Our study consistent with these study 40.74% Rotavirus (Adenovirus 13.42% 5 case is mixed infections), bacteria 24.98% and parasites 20.82% (Table 1). Most of the studies mention that mixed infections are less frequent than mono-infections , but the rate of double infections varies widely in the literature [23]. A study in Spain find the most frequent mixed infections were Rotavirus-Astrovirus (13 cases) and Rotavirus-Adenovirus (10 cases in 820 stool samples) [23] In this study Rotavirus and Adenovirus mix enfection 5 of 216 sample (2.31%) ( Table 1). This study find the highest proportion of dual infections was identified in February,March and April and seasonal occurrence of some mono-infections; infection by Rotavirus is more frequent in winter and spring . In Spain most of the cases of mixed infection occurred in autumn (26 cases in autumn, five in winter, six in spring but only two cases in summer), and no seasonal differences were detected between the different co-infections [23].
As seen in the present study Rotavirus is a most common detected antigen 40.74% for acute childhood diarrhea. These findings are consistent with other investigations in İzmir Rotavirus found 39.8% of 920 children [24]. We found Clostridium difficile infections(CDI) rate 13.88%. Some researchers find the incidence of CDI in the pediatric population appears to be increasing in US hospitals [25]. Our study has got some limitations because there is few investigations done in Erzurum before so our results are close for Rotaviruses and Adenoviruses [26].
Enteric viral pathogens were the most significant causative agents bacterial pathogens were also important contributors to pediatric diarrhea in Tripoli [27]. Resembling in Tripoli in this study viral pathogens detected 54.16% sample , bacterial pathogens detected 24.98% and parasitic agents detected 20.82% of all samples.
Entamoeba histolytica, Giardia lamblia, and Cryptosporidium parvum are considered to be the most important diarrheal agents [28][29][30][31][32]. We search Entemoeba and Giardia enfections in our study. Giardia lamblia infections are very common throughout the world and are considered one of the main nonviral causes of diarrhea in industrialized countries [32]. For many years, microscopic examination of stool samples has been considered to be the "gold standard" for diagnosis of Entemoeba histolytica, Giardia lamblia and some parasites. Recently, more specific and sensitive alternative methods (PCR,ELISA) have been introduced for all these parasitic infections. We search these parasites with microscobic examination and we found Giardia spp 8.79% and Entemoeba spp 12.03% of all samples. These numbers shows us that parasitic infections are incontrovertible cause of acute diarrhea.

Conclusıon
In coclusion analysis of viral antigens,bacterias and the parasites as a diarrheal agents in stool sample is important in 0-5 years old infants, in order to predict clinical outcome, prevent inapproprate antibiotic use and hospitalizations.