Results: Among the study population 65.83% (395/600) were male and 34.17% (205/600) were female. The sero prevalence of Human Brucellosis was found 13.33% (40/300) and 5% (15/300), respectively in risk and non-risk group of population. Out of 55 Brucella specific latex agglutination test positive samples 27.27% (15/300) were ICT positive, 5.45% (3/55) were PCR positive and 3.64% (2/55) were real time PCR positive. Among the ICT positive cases 86.67% (13/15) from risk group and 13.33% (2/15) from non-risk group of study population. Among the 3 PCR positive cases 66.67% (2/3) were ICT test positive and 33.33% (1/3) was ICT negative. All PCR and Real time PCR positive cases were found in risk group of study population.

Conclusion: No reliable data for Human Brucellosis is available in our country. However, the present study revealed that a considerable number of Human Brucellosis is present in risk group as well as non-risk group of population in both rural and urban area in Mymensingh district of Bangladesh. In our country we use only Brucella specific latex agglutination test for diagnosis of human Brucellosis. It is a non-specific test and gives many false positive results. So, it is necessary to introduce newer tests with higher sensitivity and specificity which will help to diagnose the disease rapidly and more accurately.

Keywords: PCR; ICT; PUO; Real time PCR