Abstract

Recombinant DNA technology is the desired modification of genetic material in-vivo. This technology involves inserting DNA fragments from various sources and transferring the desired DNA sequence with a vector. Manipulation in the genome of the organism can be accomplished by adding new genes or by limiting the activity of existing genes. Enzymatic digestion is used to obtain different DNA fragments. The DNA ligase enzyme is also used to insert DNA fragments into the vector. The vector is then transferred to the host cell. As the host cells proliferate, multiple copies of the vector and DNA fragment occur. In the last step, the cells carrying the transferred DNA sequence are selected and amplified. Today, most of the products used in the field of medicine, food, chemistry, biology and agriculture are produced with recombinant DNA technology. This technology has become the basis for more advanced methods that are in use today.iology

Keywords: Recombinant DNA; Usage area; Microbiology