Abstract

Vitamin D₃ and calcium are the main bone health supplements required for quality of life. The present study aimed to find out the role of Consciousness Energy Healing based vitamin D₃ and DMEM medium on bone health parameters in vitro using MG-63 cells. The parameters of bone health tested are Alkaline Phosphatise Enzyme (ALP) activity, collagen levels and bone mineralization. The Test Items (TI) i.e. vitamin D3 and DMEM medium were divided into two parts. The test samples received Consciousness Energy Healing Treatment by Krista Joanne Callas and samples were defined as the Biofield Energy Treated (BT) samples, while the other parts of each sample were denoted as the untreated test items (UT).Cell viability using MTT assay exhibited increased cell viability range from 72% to 122% with safe and nontoxic profile among test samples on MG-63 cell line. ALPwas significantly increased by 129.6%, 392% and 255.7% in the UT-DMEM+BT-TI, BT-DMEM+UT-TI and BT-DMEM+BT-TI groups, respectively at 100μg/mL as compared with the untreated group.

The level of collagen was significantly increased by 35.9% and 235.3% at 10 and 50μg/mL, respectively in the UT-DMEM+BT-TI group, while 81.7% and 197.6% at 50 and 100μg/mL, respectively in BT-DMEM+UT-TI group as compared with the untreated group. In addition, BT-DMEM+BT-TI group showed a significant increased collagen level by 184.2% and 82.3% at 50 and 100μg/mL, respectively as compared with the untreated test item and DMEM group. The percent of bone mineralization was significantly increased by 198.6% and 53.5% at 1 and 10μg/mL, respectively in the UTDMEM+ BT-TI group, while 267.1% at 1μg/mL in the BT-DMEM+UT-TI group as compared with the untreated group. In addition, BT-DMEM+BT-TI group showed a significant increased bone mineralization by 146.2%, 91.4%, and 66.8% at 0.1,1 and 10μg/mL, respectively as compared with the untreated group. Thus, the study results concluded that Bio-field Energy Treatment would be the best alternative treatment for the maintenance of strong and healthy bones and quality of life. Further, it regulates the osteoblast function and improved the level of collagen, ALP, and calcium absorption in wide range of bone dis-ordersalong with wide range of adverse bone health conditions.

Keywords: Biofield healing; Bone health; Osteosarcoma cells; Vitamin D; Bone mineralization

Abbrevations: CAM: Complementary and Alternative Medicine; NCCAM: National Center for Complementary and Alternative Medicine; MG-63: Human Bone Osteosarcoma Cells; ALP: Alkaline Phosphatase; DMEM: Dulbecco’s Modified Eagle’s Medium; FBS: Fetal Bovine Serum; FBS: Fetal Bovine Serum; EDTA: Ethylenediaminetetraacetic Acid; UT: Untreated, BT: Bio-field Energy Treated; TI: Test Item