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Significances of Bioengineering & Biosciences

Rapid Microvalve Actuated Electroosmotic Reagent Delivery to Surface-Immobilized Molecules

  • Open or Close Hao Chen*

    Biocrede Inc., USA

    *Corresponding author: Hao Chen, Biocrede Inc., Biotechnology company in Plymouth Township, Michigan, USA, Tel: (734) 678-6956; Email:

Submission: November 17, 2017;Published: January 10, 2018

ISSN 2637-8078
Volume1 Issue2


We present a micro fluidic device that allows rapid and defined delivery of discrete and homogeneous reagents or samples to allow kinetic studies of surface-tethered biomolecules. We developed an Electro Osmotic Flow (EOF) based device consisting of an asymmetric Y-junction with an incorporated fast, pressure driven valve, two embedded measurement electrodes and reservoirs. The EOF is used to circumvent kinetic limitations on reagent transport to the surface of these tethered biomolecules due to the slow diffusion across parabolic concentration gradients in conventional pressure-driven flow devices. Using Fluorescein isothiocyanate (FITC) as the pH sensitive surface-immobilized biomolecules, we show that the reagent solution can be repeatedly exchanged within milliseconds, and that by using a synchronized triggering scheme we can monitor the reaction of our sensor biomolecules to the change of the environment on a time scale of 10ms.

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