Research & Investigations in Sports Medicine

The Effect of Intermittent Capillary Blood Sampling During Load Incremented Cycling on Physiological, Psychological and Perceptual Variables

Submission: November 02, 2017; Published: February 21, 2018

Volume1 Issue5
February 2018


Introduction: The measurement of capillary blood to assess the lactate inflection point is used for health-fitness application and to predict sports performance. However, it is unknown if capillary blood sampling impacts physiological, psychological or perceptual responses during exercise testing.

Purpose: To compare the effect of capillary blood sampling on physiological, psychological, and perceptual during a load incremented cycle protocol.

Methods: A multiple observation, within subject, cross-sectional design was employed. Twenty males (20.4±2.76 years), and twenty females (22.3±3.31 years) performed two load incremented cycle ergo meter tests (Trial A and Trial B) to obtain VO2 peak (lmin-1), Ventilation (Ve) (lmin-1), VCO2 (lmin-1). VO2 (lmin-1), and HR (bmin-1) were recorded each minute. The Vpt was determined as the %VO2peak at which Ve: VO2 increased without an accompanying increase in Ve: VCO2. Trial A included capillary blood lactate (BLa) measures taken during the last minute of each stage. Trial B used an identical protocol without BLa measures. The order of administration of Trial A and Trial B were counter-balanced. The psychological/perceptual dependent variables measured during the last 30 seconds of each stage were:

1) Affect Valence (AV)

2) Felt-Arousal (FAS)

3) Rating of Perceived Exertion for the overall body (RPE- O)

4) Exercise Enjoyment (EE) and

5) Perceived intensity level (INT). A paired samples t-test was used to examine between trial differences in psychological and perceptual variables measured at the Vpt along with physiological variables.

Results: There were no significant differences in VO2 peak, HRp, TCT, and Vpt between Trial A and Trial B (p>0.05). There were no statistically significant between trial differences found for AV, FAS, RPE-O and EE measured at the Vpt. The perceived INT at the Vpt was significantly (*p<0.05) higher in Trial A than Trial B.

Conclusion: Capillary blood sampling does not appear to influence aerobic fitness variables during exercise testing or most psychological and perceptual variables. This can provide reassurance when incorporating capillary blood sampling into exercise testing protocols.

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