Consciousness Energy Healing Treatment Influenced the Physicochemical Properties of Zinc

Zinc is a vital trace element required for many physiological functions in the body [1]. The natural source of zinc are animal meat, eggs, fish, shellfish, fowl, milk, wheat (germ and bran) and various seeds like poppy, sesame, alfalfa, beans, nuts, mustard, celery, almonds, whole grains, pumpkin seeds, sunflower seeds and blackcurrant [2,3]. Zinc resides in muscle, retina, prostate, bone, skin, kidney, liver, pancreas, and particularly in the red and white blood cells. The zinc ion is the second most abundant transition metal ion in the human body, play different roles in several biological processes. It is one of the fundamental parts of more than 100 enzymes (i.e., carbonic anhydrase, carboxypeptidase, etc.) and the proteins (i.e., metallothioneins), serves as structural ions in transcription factors, nucleic acid, and protein metabolism, coordinate amino acid chains, etc. [4-7]. Other than this, zinc has many pharmacological activities includes prenatal and postnatal development, enhancement of fertility, retino-protective, immunomodulatory, antioxidant, and putative antiviral in the body [1,6-8]. It also prevents the development of many deficiency disorders, i.e., parakeratosis, hypogeusia, dysosmia, anorexia, geophagia, hypogonadism, growth retardation, Wilson’s disease, etc. [9-12]. Zinc, in combination with Liquorice (Glycyrrhiza glabra) reported with the increase leukocyte count [13]. Insufficient dietary intake, malabsorption, chronic liver, Crimson Publishers Wings to the Research Research Article


Introduction
Although zinc is essential for good quality of life, excess zinc can be harmful [16]. Zinc is soluble in acids and alkalies; insoluble in water [17]. The physicochemical properties always play a crucial role in its dissolution, absorption, and bioavailability profile of a pharmaceutical or nutraceutical compound [18]. Therefore, the researchers all over the world, paying attention to the improvement of the pharmaceutical and nutraceutical compounds. Similarly, it was experimentally observed that the Trivedi Effect ® (Biofield Energy Healing Treatment) has a huge impact on various properties of pharmaceutical/nutraceutical compounds [19][20][21][22]. The Trivedi Effect ® is a scientifically proven phenomenon in which a healer/expert can harness this intelligent energy and transfer it wherever on the planet via the possible mediation of neutrinos [23]. Every living organism generates this kind of unique energy by movement of charged particles in the body known as Biofield Energy, which is infinite, para-dimensional electromagnetic field. Biofield based energy therapies have significant outcomes against various disease [24]. National Institute of Health and National Center for Complementary and Alternative Medicine approved and comprised the energy therapy under complementary and Alternative Medicine category that has been accepted by most of the U.S. population [25,26]. The Trivedi Effect ® also been widely reported scientifically with astounding experimental data altering the characteristic properties of the several non-living and living objects i.e. metals and ceramics [27,28], organic compounds [29,30], nutraceuticals/pharmaceuticals [31][32][33], microorganisms [34,35], and crops [36,37]. Therefore, this study was performed to determine the impact of the Trivedi Effect ® on the physicochemical properties of zinc using sophisticated analytical techniques.

Chemicals and reagents
The test sample zinc powder was purchased from Sigma Aldrich, USA, but the other chemicals were purchased in India.

Consciousness energy healing treatment strategies
The test sample of zinc powder was divided into two parts. One part of zinc powder sample was not received the Biofield Energy Treatment called as a control sample. But, the other part of the test sample was received the Trivedi Effect ® -Consciousness Energy Healing Treatment remotely in standard laboratory conditions for 3 minutes by the famous Biofield Energy Healer, Alice Branton, USA, and known as the treated zinc powder. The control zinc powder was treated with a "sham" healer who is ignorant about the Biofield Energy Treatment. After that, both the samples were kept in sealed conditions and characterized using modern analytical techniques.

Characterization
The X-ray diffraction (PXRD) analysis of zinc powder sample was conducted with the help of Rigaku MiniFlex-II Desktop X-ray diffractometer (Japan) [38,39]. The crystals size was calculated using the Scherrer's formula (1)

G = kλ/βcosθ
Where G: crystallite size in nm, k: equipment constant, λ: radiation wavelength, β: full width at half maximum, and θ: Bragg angle [40]. The particle size distribution (PSD) analysis was conducted with the help of Malvern Mastersizer 2000 (UK) using the wet method. Similarly, the thermogravimetric analysis (TGA) of zinc metal powder was performed with the help of TGA Q50 TA instruments [41,42]. The % change in PXRD peak intensity, crystallite size, particle size, specific surface area, weight loss and the maximum thermal degradation temperature of the treated zinc powder was calculated compared with the control sample using the following equation 2: *The percentage change in the peak intensity, and crystallite size of treated zinc compared to the control sample.  (Table 1). All the peak intensities of the treated zinc powder were significantly increased from 7.74% to 112.59% compared to the control sample. The crystallite sizes of the treated zinc powder were significantly altered, ranging from -42.43% to 84.50% compared to the control sample. Generally, the average crystallite size of the treated zinc (472.22nm) was significantly decreased by 23.86% compared with the control sample (620.22nm).

Powder x-ray diffraction (PXRD) analysis
The variations in the crystallite sizes and peak intensities indicated the change of the crystal morphology of the Biofield Energy Treated zinc compared to the control sample. The peak intensity of each diffraction face on the crystalline compound alterations according to the crystal morphology [43] and alterations in the PXRD pattern provide the evidence of polymorphic changes [44,45]. Thus, it was concluded that the Trivedi Effect ® -Consciousness Energy Healing Treatment probably produced the new polymorphic form of zinc through the Biofield Energy via neutrino oscillation [23]. Different polymorphic forms of pharmaceuticals have the significant effects on the drug performances because of their thermodynamic and physicochemical properties like melting point, energy, stability, and especially solubility, are different from the original form [46,47]. Thus, it can be anticipated that the treated zinc would be better in designing pharmaceutical and/or nutraceutical formulations containing zinc.

Particle size analysis (PSA)
The particle size distribution analysis of both the powder samples was performed. The PSD curve of the treated zinc powder was meaningfully different compared to the control sample. One extra peak was observed in the case of the treated zinc compared to the control zinc ( Figure 2). The particle size values in the Biofield Energy Treated zinc were significantly decreased at d 10 , d 50 , d 90 , and D (4,3) by 28.10%, 97.98%, 97.43%, and 97.01%, respectively compared to the control sample ( Table 2). The specific surface area of Biofield Energy Treated zinc powder (0.517 m 2 /g) was significantly increased by 120% compared with the control sample (0.235 m 2 /g). Hence, it can be assumed that the Biofield Energy Treatment might be acting as an external force for reducing the particle size; hence increased the surface area of zinc. Reducing the particle size of pharmaceutical compounds increase the surface area and improve the solubility, dissolution rate, and bioavailability in the physiological system [41,48]. Zinc is insoluble in water [17]. Thus, it is assumed that the treated zinc might offer better bioavailability compared with the control sample.  * The percentage change in the particle size distribution and surface area of the treated zinc compared to the control sample. Figure 3: TGA thermograms of the control and treated zinc.

NTNF.000586. 4(3).2019
The TGA thermograms of both the samples showed two steps of thermal degradation process (Figure 3). The total weight loss in Biofield Energy Treated zinc was significantly increased by 196.22% compared with the control sample (Table 3). Therefore, the residue amount was significantly decreased by 46.18% in the Biofield Energy Treated zinc compared to the control sample ( Table  3). The DTG thermograms of the control and Biofield Energy Treated zinc powder also exhibited two peaks (Figure 4). The T max of 1 st and 2 nd peaks in the Biofield Energy Treated zinc was significantly decreased by 5.61% and 10.22% compared with the control sample (Table 3). Overall, TGA/DTG revealed that the thermal stability of the Biofield Energy Treated zinc was significantly decreased compared with the control sample.

Conclusions
The Trivedi Effect ® -Consciousness Energy Healing Treatment showed significant effects on the physicochemical properties of zinc powder. The PXRD peak intensities of the Biofield Energy Treated zinc were significantly increased from 7.74% to 112.59% compared to the control sample. Similarly, the crystallite sizes of the Biofield Energy Treated zinc were significantly altered, ranging from -42.43% to 84.50% compared to the control sample. However, the average crystallite size of the Biofield Energy Treated zinc was significantly decreased by 23.86% compared to the control zinc. The particle size values in the Biofield Energy Treated zinc powder were significantly decreased by 28.10% (d 10 ), 97.98% (d 50 ), 97.43% (d 90 ), and 97.01% {D (4,3)} compared to the control sample. Thus, the specific surface area of the Biofield Energy Treated zinc powder was significantly increased by 120% compared with the control sample. The total weight loss was significantly increased by 196.22%; however, the residual amount was significantly increased by 46.18% in the treated zinc compared with the control sample.

NTNF.000586. 4(3).2019
The T max of 1 st and 2 nd peaks the Biofield Energy Treated zinc was significantly decreased by 5.61% and 10.22% compared with the control sample. The Consciousness Energy Healing Treatment might be responsible for a new polymorphic form of zinc, which would be more soluble, and bioavailable compared with the control sample. The Trivedi Effect Treated zinc would be very useful to design better nutraceutical/pharmaceutical formulations that might offer better therapeutic response against parakeratosis, hypogeusia, dysosmia, anorexia, geophagia, hypogonadism, growth retardation, Wilson's disease, etc. Other than this, the Biofield Energy Treated zinc powder could be beneficial for the prenatal and postnatal development, enhancement of fertility, retino-protective, immunomodulatory, antioxidant, and putative antiviral activity in the body. The lowering of the thermal behavior it could be useful for the industrial applications for galvanization, anti-corrosion agent, preparations of alloys, chemical reactions, etc.