Immune-Metabolic Stress in Purulent-Inflammatory Diseases of the Skin and Soft Tissues

It is known that metabolic processes include free radical oxidations (ROS), which provide cells with energy to prepare plastic material, participate in the metabolism of carbohydrates, lipids, proteins, nucleic acids. ROS leads to the formation of primary (diene conjugate [DC], ketodienes [KD]) and secondary (malonic dialdehyde [MDA], bityrozine crosslinks [BC], Schiff ’s bases [SHB]) and other products that have a positive or negative effect on the biomembrane cells. For the leveling of ROS processes in the body, an endogenous antioxidant defense system (AOD) is developed, which consists of enzymatic and non-enzymatic links. The first group includes superoxide dismutase (SOD), catalase (C), peroxidase (P), glutathion peroxidase (GP), glutathione reductase (GR); to the second, glutathione (GL), alpha-tocopherol (vitamin E, VE), plasma antioxidant activity (AOA), total thiols, non-protein thiols, protein thiols (TT, NPT, PT), etc. [1,2]. It has been established that ROS products mainly implement a suppressive and AOD a modulating effect on the immune reactivity of patients [3]. At the same time, the direct link between immune-metabolic parameters, recognized by all authors is practically not documented [4-7].


Introduction
It is known that metabolic processes include free radical oxidations (ROS), which provide cells with energy to prepare plastic material, participate in the metabolism of carbohydrates, lipids, proteins, nucleic acids. ROS leads to the formation of primary (diene conjugate [DC], ketodienes [KD]) and secondary (malonic dialdehyde [MDA], bityrozine crosslinks [BC], Schiff's bases [SHB]) and other products that have a positive or negative effect on the biomembrane cells. For the leveling of ROS processes in the body, an endogenous antioxidant defense system (AOD) is developed, which consists of enzymatic and non-enzymatic links. The first group includes superoxide dismutase (SOD), catalase (C), peroxidase (P), glutathion peroxidase (GP), glutathione reductase (GR); to the second, glutathione (GL), alpha-tocopherol (vitamin E, VE), plasma antioxidant activity (AOA), total thiols, non-protein thiols, protein thiols (TT, NPT, PT), etc. [1,2]. It has been established that ROS products mainly implement a suppressive and AOD -a modulating effect on the immune reactivity of patients [3]. At the same time, the direct link between immune-metabolic parameters, recognized by all authors is practically not documented [4][5][6][7].
The study aimed to validate the objective assessment of typical changes of the laboratory status using non-specific methods and to document functional relations between variations of immune and metabolic parameters (immune metabolic stress) in different forms of purulentinflammatory diseases of the skin and soft tissues.
All patients were divided into 4 randomized groups of 19-25 people each by sex, age, disease severity. 30 healthy donors form the control group. In the acute period of the disease, routine hematological markers of inflammation were determined in patients. An immune examination identified populations and subpopulations of lymphocytes, characterized phagocyte absorbency using flow cytofluorometry (HAVIOS Beckman Coulter), using monoclonal antibodies (CYTO-STATtetraCHROM). The assessment of the oxygen-dependent metabolism of neutrophils was carried out with a nitro-blue tetrazolium test (NBT), the content of circulating immune complexes -spectrophotometrically according to Haskova with polyethylene glycol, the analysis of the immunoglobulin isotypes (IgG, IgA, IgM) was performed using the monospecific antiserum manufactured by Biosystems (Spain) by the turbidimetric method on a semiautomated biochemical analyzer Screen Master Plus (Hospitex Diagnostics S.A., Sesto Florentino, Italy) at the wavelength of 340mm and cytokinesenzyme-linked immunosorbent assay using "Protein Circuit" kits [3]. The parameters of ROS and AOD were determined using the methods of UV-spectrophotometry, fluorescence, reaction with 2-thiobarbituric acid, etc. [1,2].
Calculation of the coefficient of diagnostic value carried out according to the formula: where δ 2 1 and δ 2 2 -mean root square deviations, M 1 , M 2 -mean values of the parameters of the compared groups.
The interpretation of the key laboratory tests: the greater the difference between the coefficient module and the normal level, the higher the information significance of the parameter.
Key indicators are formalized into standard immune system disorders formula (ISDF) and metabolic disorders formula (MDF). Statistical significance of correlations between metabolic and immunological parameters was characterized using correlation coefficients > 0.6 [3].
For an integral assessment of the hematological, immune, and metabolic parameters, the ranking was used on a scale: insignificant (rank III) with a significant change in the value of the indicator in 0-33% of patients, medium (rank II) -34-66%, significant (rank I) -more than 66% [4,5].  The frontal analysis of variation of hematologic inflammatory markers devoted to Table 1.

Result
According to the frontal analysis of the reaction of hematological witnesses of inflammation in different nosological forms, the purulent-inflammatory diseases (PID) were stimulating in the range from 57 to 86-100%, that is, not statistically significant. When considering variations from the normal level of the parameters grouped by separate links of immunity, the following changes were established. The defining dynamics of T-dependent parameters in patients with DP, PSTI, PSTI+TE was suppression in the range of 50-62.5%. As in the previous, allergization of patients caused a different reaction, a parity change of index of the T-link of immunity -37% ~ +37.5%. At the same time, in all patients, 50-100% was activated by humoral immunity and 50-83% in phagocytic depressed. Variations in the level of interleukins were observed in the range of 16.7 -33.3%.
The reactions of the metabolic immunity terms turned out to be more unambiguous (see Tables 1 and Table 2). The parameters of free radical oxidation (ROS) of substrates in patients revealed that 60-100% were stimulated, and the antioxidant system was predominantly 50-62.5% suppressed (see Table 2). So, in patients with DP, the levels of malonic dialdehyde, ketodienes, Schiff bases were stimulated, as with PSTI and PSTI+TE, it concerned MDA, DK, KD, SHB, BC. At DP+SA it concerned MDA, DC, SHB, BC. Concerning the components of the antioxidant system in all patients, the trend of suppressing the antioxidant activity of blood plasma was predominant and less pronounced at common thiols in patients with PSTI, DP+KA, PSTI+TE. At non-protein thiols in patients with DP+SA and PSTI+TE, but regarding catalases in patients with DP. Variations in the concentration of vitamin E, superoxide dismutase, and protein thiols were changed in different directions.
The ranking method for evaluating the indicators revealed the maximum changes in all summarized (column 2) laboratory parameters in patients with a combination of pathological processes: PSTI+TE and DP+SA (1) and averages (2) with monopathologies: DP and PSTI. At the same time, in all cases, the general stimulation of the parameters (column 3) was moderate (2), and the suppression (column 4) was significant only in patients with PSTI+TE (2).
The determination of the final reducing algorithm of all significant differences in the laboratory parameters studied for individual nosoforms of purulent-inflammatory diseases was as follows: purulent soft tissue infection complicated by true eczema, deep pyoderma, aggravated by skin allergies; and monopathology, involving purulent infection of soft tissues and deep pyoderma.
The results of the determination of the signaling immune and metabolic laboratory parameters of patients with different purulent-inflammatory diseases using the coefficient of diagnostic value are presented in Table 4. According to the standard immune system disorders formula (ISDF) in patients with DP the accumulation of 3 degrees of auto-aggressive circulating immune complexes, cytotoxic natural killer cells, proinflammatory cytokine -interleukin-6 was decisive. In patients with another variant of PSTI, the leading group had the ultimate increase in the content of T cytotoxic lymphocytes of suppressors and proinflammatory IL-8 against the background of a decrease in the level of B-cells of moderate severity. In patients with a combined pathology, DP+SA, the leading ones turned out to be significant eosinophilia, an excess of B-cells, and proinflammatory tumor necrosis factor-alpha. In the case of purulent soft tissue infection+ true eczema, an imbalance of regulatory subpopulations is formed T-helper cells and T-cytotoxic suppressors against the background of the accumulation of CIC in blood serum, 3 degrees in all cases. Table 4: Signaling parameters of the key of immune-metabolic disorders formulas and their correlation indicators in patients with deep pyoderma and purulent soft tissue infection.

Metabolic Disorders Formula (MDF) Correlations
Deep pyoderma (DP) n=25 The definition of strong, with a correlation coefficient of >0.6, reveals the functional unity of the consistent dynamics of immune and metabolic parameters in patients with different options for purulent-inflammatory diseases in the acute stage (see Table 4).
From Table 4 it follows that in patients with deep pyoderma in the acute stage, the key parameter of the immune system disorders formula (ISDF) reveals an increased concentration of circulating immune complexes was positively consistent with the Schiff's base level (the ROS system) and negatively with the AOD enzyme factor ceruloplasmin. At the same time, an overestimated amount of cytotoxic natural killer cells was directly associated with antioxidant activity (AOD) of blood plasma, and the content of interleukin 6 was also associated with ketodiene related to the processes of free radical oxidation of lipids and proteins.
In patients with purulent soft tissue infection, the dynamics of the signaling parameters were an increased number of T cytotoxic suppressors directly correlated with ROS factors, malonic dialdehyde, a deficiency of B-lymphocytes with non-enzymatic and enzymatic mechanisms of the antioxidant system AOD and Catalase.
Combined pathology of DP with SA caused the modification of not only the composition of typical ISDF, MDF but also the associations of their components belonging to the ROS system, various AOD mechanisms. Thus, eosinophilia was directly related to the concentration of diene conjugates and back to AOD. The accumulation of B-lymphocytes is also variable with the level of vitamin E and Schiff's bases. An excess of the proinflammatory TNF-α was positively associated with bitirozine crosslinks and negatively with malonic dialdehyde and common thiols.
The combination of PSTI with TE, in turn, contributed to a consistent positive dynamics of the regulatory subpopulation T-helpers with MDA, DC, and negative with superoxide dismutase. Also, T cytotoxic suppressors were negative with protein thiols, ceruloplasmin. CIC multidirectional were tied with BС and AOD.
Thus, there are features of changes in hematological, immune, and metabolic parameters in patients suffering from various mono and combined purulent-inflammatory diseases of the skin and soft tissues with the possibility of using these data after mathematical processing for diagnostic purposes are established.

Conclusion
During the examination of more than 100 patients suffering from 4 types of mono-and combined purulent inflammatory diseases of the skin and soft tissues, diagnostic changes were established in general indicators, detailed to the individual links of immunity, as well as the primary and secondary metabolic parameters of free radical oxidation of lipids, proteins, enzymatic and non-enzymatic mechanisms of the antioxidant system with the formalization of supporting indicators in diagnostic formulas. A direct and inverse correlation has been revealed between the key immune-metabolic markers of patients, indicating the functional unity of the factors of immune-oxidant stress.