Oil Extraction from Two Species of Sesame ( Sesamum Indicum ) and the Chemical Composition of the Meal

Subject description: the objective of this work and to exploit the product and the by-Product of sesame grown in Mali. Method: Two methods are used, one mechanical and the other chemical, to evaluate the yield and the quality of the product. Result: After extracting the oil (fat: MG) with a compress, the oil cake still contains 31.95% of it, which must be exploited either by improving the extraction method, or by using it in animal feed. Besides whatever the chemical used for the extraction, the result is almost the same 51.59% and 50.62% respectively with benzene and petroleum ether. On the other hand, the average fat content (MG) differs from the Malian species (ESE1) to that marketed (ESP) in Algeria, as well as the mineral content. However, the protein content is the same 18.5% DM. The two elements, proteins and minerals increase after extraction of MG. Meal is a free source of fat for animals. The fiber content before extraction was 19.75% then increased in the cake to 35.78%. This rate can be improved for good digestibility. Conclusion: The oil yield by the mechanical method can be improved by increasing the extraction pressure.

It is cultivated in Asia, America, Europe and Africa. Not very demanding in terms of water and manure and it adapts to various types of climates. Its production has various uses. The production area in Africa is expanding under the pressure of "demand on the international market, greater than supply" [5].
Asia provides 70% of world production; America and Europe respectively at 4.98 and 0.038%. Africa contributes 15% [2]. [6] revealed the presence of genetic variability in sesame for seed yield significantly and stated that crosses of parental genotypes have the potential to increase total seed yield of sesame.
Nutritional quality and oxidative stability are two main factors in the evaluation of edible oils. The quality characteristics and composition of sesame oils prepared using a household electric oven suggests that a high-quality product would be obtained by roasting for 25 min at 160 or 180 °C, 15 min at 200 °C. and 5 min at 220 °C compared to other samples [7].
To learn more about the yield and quality of the oil produced by mechanical and chemical methods as well as the exploitation of the residue in ruminant feed; we have undertaken to conduct the research of nutritional value through the physical and chemical composition of the oil and its cake of two cultivars of Sesamum indicum one of Malian origin and the other of Sudanese origin.

Origin of plant material
The first species, Sesamum samples studied, comes from Mali from the Koulikoro region located east of Bamako. The second species comes from the Algerian market (Sudan).

Extraction and dosing
The plant material (sesame seeds) was subjected to oil extraction and chemical analysis before and after extraction. The oil was subsequently analyzed. Figure 1 brings together all the stages of the experimental approach followed. Oil extraction from sesame seeds By the solvent: From the flour resulting from the grinding of sesame seeds, we proceeded to extract the oil using the soxhlet extractor. The solvents used are n-hexane, petroleum ether and benzene. For each sample of about 10g we have used a sufficient amount, the cup is not large so that once it is empty, we fill it. The extractions were carried out for 4h with n-hexane and petroleum ether and 3h 30 benzene at a temperature of 90 °C and 100 °C. The soxhlet extraction used is composed of 3 essential parts: The ball or a glass.
ii. The cartridge column and the refrigerant.
iii. A water cooler.
By compress: After mechanical grinding ( Figure 1) of sesame seeds (200g) using a screw mill (Figure 2), the paste obtained is manually pressed with a compress (tulle) in a glass (Figure 3 & 4). The oil obtained is measured by test tube to assess the yield of the seeds weighed beforehand.

Seed and meal analysis process
Crushing of samples before analysis. Depending on the fineness of the ground material obtained (fibers>1mm or fine dust).
Chemical analyzes of sésame flour and meal: In order to know the nutritional value of the flour and meal of the two species studied, we carried out the chemical analyzes according to the intentional ISO standards reported in the following book of fodder analyzes [8] Détermination of Dry Matter (DM) / water content: Put empty weighing vessels, previously dried in the oven, for at least 1 hour in a desiccator. After cooling, tare to the nearest 0.1mg for each weighing vessel. Let M 0 be their mass. Weigh into the vessels, to the nearest 0.1mg, about 3g of ground sample. Let PE be the test socket. Spread the test sample evenly with a slight rotary motion. Place the full weighing vessels in an oven at 103 °C for 24h. The timer will be started as soon as the oven has reached the desired temperature again. After 24 hours, take out the weighing vessels and allow them to cool in a desiccator. Weigh them to the nearest 0.1mg. Let M 1 be their mass.

Determination of ash/mineral content (MM):
The mineral matter is obtained after destruction of the organic matter by incineration. Put the nickel crucibles which were used to determine the dry matter for at least 1 hour at 200 °C in the muffle oven and then calcine for 2 hours at 550 °C. The stopwatch will be started as soon as the oven has reached the desired temperature.

Crude Fiber (CF) WENDE method:
The sample is treated with a 1.25% solution of sulfuric acid and then with a 1.25% solution of sodium hydroxide. Each treatment lasts 30±1 min. The residue is each washed with hot water and dried. Finally, the residue is calcined for 1 hour at 530 °C.
The calculation of crude cellulose (CF): Obtaining the residue (P1) after 24 hours in the oven.
Determination of total nitrogen: kjeldahl method: In a plant or even in a soil, nitrogen can be found in mineral and organic form (proteins, phospho-amino-lipids); to dose it in its entirety, it is necessary to destroy the organic compounds so as to obtain all the nitrogen in the same mineral form. Mineralization is carried out for this. The nitrogen is then dosed by acid-base assay. B: Weight of the balloon or glass in g.
C: Weight of the initial sample in g.

Physical analysis:
The oils (obtained by the soxhlet method or by pressing with a compress) have undergone three tests:

i.
Taste: a few drops of oils from each extraction are deposited in the tongue and turned in the mouth.
ii. Color: visually, the color of each variety is determined.
iii. Touch: with two fingers, index and push the oil is rubbed to assess the degree of roughness.

Result and Discussion
The two cultivars studied (Table 1)  properties of sesame seeds vary according to several parameters including the aspect: genetics, maturity of the seed, harvesting and storage conditions. [7], showed in their study of the genetic diversity of Sesamum indicum, the varieties offer more oils than populations.
The Malian species is heavier so the seedling dose will be lower than the species marketed in Algeria. It is an important cash crop for small farmers and farmers [9,10].
For both species the biological extraction method gives a cloudy appearance to the oil which requires its purification by centrifugation or decantation. On the other hand. Solvent extraction, the oil of the species is creamy yellowish. According to [11], the design of an extraction system meeting predefined objectives requires the choice of solvents essentially according to their polarity, the aim of which is to optimize the extraction Manual extraction (with the screw and tulle mill) of 200g of Malian sesame seeds offers a volume of 75ml, is a yield of 37.5%. On the other hand, the extraction with the petroleum ether solvent of 10.18g of Malian sesame acquires 5.25g of oil, is a yield of 51.55%.
Although the extraction of sesame oil with the screw and tulle mill gives a lower fat value than that obtained by the soxhlet method, the quality of the latter is better and will bring in more money. It is more in demand for human consumption. The cakes obtained after extraction can be used to feed livestock. Thus, the product and byproduct of organic sesame do not carry a risk of toxicity.
According to the species after extraction with a compress, the oil cakes still contain on average 29.28% fat and 46.26% fat respectively Malian species and species from the Algerian market (Table 2). This must be exploited either by improving the extraction method (example using a press), or by using oil cakes in animal feed. In the literature, the oil cake of sesame oil extraction by pressure still contains 8-10% of residual oil [12].
In addition, whatever the solvent used for the extraction, the result is almost the same 51.59% and 50.62% respectively with benzene and petroleum ether ( Table 2). According to some authors, Wang et al. [13] the extraction yields of sesame oils were largely dependent on the extraction solvent, there is a solute-solvent interaction. Polar solvents are considered to be bio-renewable promoters for the extraction of oils. Their action manifests itself through the rupture of cell walls allowing a more in-depth extraction of cellular content [13,14].
However, the average fat content in the seeds differs from one species to another. The Malian species (ESE1) records 51.59% and that marketed in Algeria (ESP2) allocates 49.91% (Table 2). These results are in agreement with the observations reported in the literature ranging between 43 and 58% [15,11].
The literature shows that there is not much variability in the ash content of sesame seeds, between 3.7% and 6.5% [15]. Several other authors claim that the content depends essentially on the growing medium of the plant. Our results vary between 5.57% DM and 10.51% DM respectively for the Malian species and the species of the Algerian market. These proportions increase in the meal of the first species and decrease in the second species ( Table 2).
The protein content in sesame seed is almost the same 17.7 and 18.4% so it is a species-specific value. However, various authors have shown that the average protein content of sesame seeds is between 16.3% and 25% [15]. Thus, the improvement of the content in this parameter is possible by selection.
After extraction of the oil, that is to say in the oil cakes, the protein levels change in the Malian species (from 18.44 % to 23.33% expressed in % DM), in the species imported in Algeria increased

MCDA.000698. 8(5).2021
by (17.7% to 18.12% expressed in %DM), which is a free source for animal feed. Non-enzymatic browning can lead to a decrease in nutritional properties with a decrease in the digestibility of proteins and in the solubility of the product leading to the loss of amino acids, vitamins and development of the undesirable flavor with change in texture and increase in acidity [16].
The average moisture content of the seed meal of the two species is the same due to the presence of the seed coat in the seed. The disappearance of the latter during grinding increases the humidity of the oil cakes from 3.99 and 3.16 to 4.26% (Table 2). Despite the similarity of the color of sesame seeds, their moisture content is different [11]. [17] Found in seed varieties originating in Nigeria a humidity of 4.73±0.02% of black color, 4.12±0.01% for seeds of brown color and 4.14±0.05 % for white seeds. [18] reported that the moisture content of different cultivars from different countries was between 3.65 and 5.60.
Water contents between 5.3% and 8% have been reported by [19]. Nigerian sesame with an average water content of 3% is considered a dry product. This could be due to the warm climate and the mode of drying and storage [19].
The fiber content before and after fat extraction is different. Thus, in both species, oil cakes have a higher proportion of fibers (CF) than sesame flour before extraction; in the Malian species increased from 19.75% to 35.78% while the species imported into Algeria increased from 14.22% to 17.5% (Table 2).
Everything depends on the ration of the animal this rate can be improved for good digestibility when already a rate of 30% is considered good for rumination according to [20,21].

Conclusion
The physico-chemical analysis of the sesame seeds and the physical sesame oil of the two cultivars studied reveals a very interesting fat content of 49.91% and 51.59% with the soxhlet method. With mechanical extraction, the quantity is much less than 32% but can increase after improvement of the extraction method. However, this even small amount will cost more than that which will carry traces of solvent is a source of currency. In any case, sesame oil is an economic source not to be overlooked by the Malian farmer. It is within reach of the farmer or a group of farmers. The organic extraction method is easy to carry out by the Malian woman, whose oil can be used in cooking. The meal obtained from the extraction can be used in animal feed, and in the preparation of mixtures of concentrate in industries which import soybean meal.