Abstract

The study was aimed tocharacterized and determinethe antibiotic sensitivity pattern of bacteria isolated from semen of male patients with infertility attending Murtala Muhammad Specialist Hospital (MMSH) Kano, Nigeria. Two hundred (200) Semen specimens were collected from males with infertility attending the clinic and General out Patient Department of MMSH Kano. The seminal fluids were diluted with sterile saline, centrifuged and cultured on Nutrient agar, Blood agar, Chocolate agar and MacConkey agar then incubated aerobically and in 5% CO2at 37°C for 24 hours for the isolation of pathogenic microorganism. Isolates were identified based on Gram’s staining, biochemical tests and API 20E Test. The result shows a total of seventy six (76) isolates were obtained, Staphylococcusaureus was found to have the highest occurrence of 31 (40.79%), whereas the least was found to be Mycoplasma species, 1(1.32%). Other microorganisms encountered include; Coagulase negative Staphylococcus species14 (18.42%), Escherichia coli 11(14.47%),Klebsiella pneumonia 6(7.89%), Proteus mirabilis 5(6.58%),Pseudomonas aeruginosa 6 (7.90%) andNeisseria gonorrheae2(2.63%). The isolates were most sensitive to ciprofloxacin and ofloxacin. Ceftrizone and cefuroxime however showed superior activity against most of the isolates. The activity of the Tetracycline and Augumentin against almost all the isolates was not appreciable. Cotromethazole and Gentamicin showed moderate activity against most of the isolates and no activity was observed on Mycoplasma and Neisseria gonorrhoeae against most of the antibiotics.

Keywords: Antibiotics; Bacteria;Infertility;Semen;Sensitivity pattern